GPCR Protein - V2R-GS Analyses

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DNA Cloning

• Quick-start services for gene synthesis and cloning (only the gene sequence or amino acid sequence of POI required)
• Codon optimization
• Select appropriate vector and expression system.

Recombinant Protein Expression & Purification

• Customer sets preference for protein expression system (bacteria, yeast, insect, or mammalian).
• Customizable modifications (i.e. protein fusion tags).
• Select purification methods (i.e. AC, IE, or SEC).
• Detergent screening services (ensure the POI is folded correctly and retaining biological activities).

CryoEM Structural Analysis

• Negative staining method to confirm protein homogeneity and particle integrity. 
• Flash-freezing POI or its associated complex to stabilize conformation.
• Explore appropriate grids, detergents and freezing conditions.
• RELION, cryoSPARC software to process data.