- Quick-start services for gene synthesis and cloning (only the gene sequence or amino acid sequence of POI required)
- Codon optimization
- Select appropriate vector and expression system.
Recombinant Protein Expression & Purification
- Customer sets preference for protein expression system (bacteria, yeast, insect, or mammalian).
- Customizable modifications (i.e. protein fusion tags).
- Select purification methods (i.e. AC, IE, or SEC).
- Detergent screening services (ensure the POI is folded correctly and retaining biological activities).
CryoEM Structural Analysis
- Negative staining method to confirm protein homogeneity and particle integrity.
- Flash-freezing POI or its associated complex to stabilize conformation.
- Explore appropriate grids, detergents and freezing conditions.
- RELION, cryoSPARC software to process data.